Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.
نویسندگان
چکیده
منابع مشابه
The nucleotide sequence of the genes, fanE and fanF of Escherichia coli K99 fimbriae.
K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing seve...
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The erythrocyte receptor for Escherichia coli K99 fimbrial adhesin was isolated from equine erythrocytes and characterized as Neu5Gc-alpha(2----3)-Galp-beta(1----4)-GLcp-beta(1----1)-Ceramide. This glycolipid acted as the receptor for K99 by four different experimental approaches: inhibition of equine erythrocyte hemagglutination by preincubation of K99-positive bacteria or purified K99 fimbria...
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Single-chain antibodies (scFv) are small, recombinant proteins in which variable domains from immunoglobulin heavy and light chains are physically linked with a flexible peptide. They are monovalent and lack the heavy chain constant domins for activation of complement and binding to cellular receptors. However scFvs can possess biological activity by binding to and inhibiting the action of thei...
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Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-n...
متن کاملIn vivo emergence of enterotoxigenic Escherichia coli variants lacking genes for K99 fimbriae and heat-stable enterotoxin.
Neonatal pigs were inoculated with porcine enterotoxigenic Escherichia coli 431, which carries genes for K99 fimbriae and STaP enterotoxin. Colonies of strain 431 were recovered from feces of pigs for up to 17 days after inoculation and tested for hybridization with gene probes for K99 and STaP. Variants of strain 431 that did not hybridize with the probes were considered to have lost the genes...
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ورودعنوان ژورنال:
- The Japanese journal of veterinary research
دوره 41 2-4 شماره
صفحات -
تاریخ انتشار 1993